Step 1 Obtain a human gene sequence with its intron/exon structural information from Ensembl "Exon View", inclusive of all sequence structure information in the region of your interest, as shown in this image, to input into the Expeditor2 to start with.
Step 2 Exam the template sequence formed, edit if necessary. This is a step at which you can launch the "Expeditor Consensus Tool" to obtain a consensus sequence of your target (from multiple species), as a substitution of the exon to which there is no EST equivalents from your species.
Step 3 Design primers on the template made. With Expeditor2, you can map your primers against the template sequence for their locations.


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